
The game contains characters seen in the first five parts of the series.

In addition to battle segments, Stardust Shooters includes animated sequences and character models featuring the entire JoJo cast from the animated parts. Yellow and Black Strikers have advantages and disadvantages against each other). Medal Strikers also possess different colors, each color has an advantage and a disadvantage over specific others (Red has an advantage over Green, Green over Blue, and Blue over Red. Like some card battler games, players must take into account a medal's available health and special attacks as well as take advantage of combo systems that will chain stronger attacks together. Combat against enemies is done using Medal, particularly "Striker" Medals, each of which represents one character and their ability set.

The game uses the touchscreen as the main form of interaction, allowing the player to input commands using their fingers.
